CD1b antibody (CF®740)

Catalog No. ABIN7650932

The Mouse Monoclonal anti-CD1b antibody has been validated for FACS. It is suitable to detect CD1b in samples from Human.

Quick Overview for CD1b antibody (CF®740) (ABIN7650932)

Target: CD1b (CD1B)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD1b antibody is conjugated to CF®740

Application: Flow Cytometry (FACS)

Clone: RIV12

Product Details anti-CD1b Antibody (CF®740)

Purpose: CD1b(RIV12), CF740 conjugate

Characteristics: The mouse monoclonal antibody recognizes CD1b, a 44 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells. The CD1 multigene family encodes five forms of the CD1 T-cell surface glycoprotein in human, designated CD1A, 1B, 1C, 1D and 1E. CD1, a type 1 membrane protein, has structural similarity to the MHC class I antigen and has been shown to present lipid antigens for recognition by T lymphocytes. Constitutive endocytosis of CD1B molecules and the differential sorting of MHC class II from lysosomes separate peptide- and lipid antigen-presenting molecules during dendritic cell maturation. CD1B is also expressed in interdigitating cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human peripheral lymphocytes

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL

Comment:

Positive Control: MOLT4 cells. Human thymus.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for CD1b

Target: CD1b (CD1B)

Alternative Name: CD1b

Background:

Synonyms: A1 domain, CD1B, Cortical thymocyte antigen CD1B, hTa1 thymocyte antigen, Ly38, T cell surface glycoprotein CD1b, T6/Leu6

Gene Symbol: CD1B

Tissue Expression: Antigen presenting cells

Molecular Weight: 49 kDa

Gene ID: 910

UniProt: P29016